A phylogenetic tree of 17 AMIGO proteins found in Xenopus, chicken, rat, mouse, human, zebrafish, and medaka was generated by the Clustal multiple-sequence alignment program (on the right). Identity of the amino acid sequences is indicated in the table. A, alignment of the amino acid sequences of the zebrafish Amigos with 14 AMIGO proteins found in other species (on the left). Of the Kv2.1 potassium channel to form functional neural circuitry that controls locomotion.ĪMIGO proteins in different species, expression of the amigos in zebrafish, and production of antibodies to detect Amigo1 of zebrafish. We suggest that Amigo1 is essential for the development of neuralĬircuits of zebrafish, where its mechanism involves homophilic interactions within the developing fiber tracts and regulation Inability of the larvae to perform normal escape responses. Analysis of differentiated neural circuits reveals defects in the catecholaminergic system.Īt the behavioral level, the disturbed formation of neural circuitry is reflected in enhanced locomotor activity and in the Knockdown of amigo1 expression using morpholino oligonucleotides impairs the formation of fasciculated tracts in early fiber scaffolds of brain.Ī similar defect in fiber tract development is caused by mRNA-mediated expression of the Amigo1 ectodomain that inhibits adhesion Here we have cloned all three zebrafish amigos and show that amigo1 is the predominant family member expressed during nervous system development in zebrafish. Previous in vitro studies have suggested a role as homophilic adhesion molecules in brain neurons, but the in vivo functions remain unknown. Immunoglobulin-like domain in their extracellular moieties. The Amigo protein family consists of three transmembrane proteins characterized by six leucine-rich repeat domains and one
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